为防止在使用多个屏幕的情况下窗口被打开在两个屏幕之间，可以使用以下代码示例将窗口限制在指定屏幕上：

import wx

class MyFrame(wx.Frame):
    def __init__(self):
        wx.Frame.__init__(self, None, wx.ID_ANY, "My App")
        
        # 获取屏幕分辨率
        screen_width, screen_height = wx.GetDisplaySize()
        
        # 获取主屏幕位置和大小
        primary_screen = wx.ScreenDC().GetWindow()
        primary_screen_pos = primary_screen.ClientToScreen(wx.Point(0, 0))
        primary_screen_size = primary_screen.GetSize()
        
        # 如果窗口大小大于主屏幕大小，将其大小设置为主屏幕大小
        if self.GetSize()[0] > primary_screen_size[0] or self.GetSize()[1] > primary_screen_size[1]:
            self.SetSize(primary_screen_size)
            
        # 如果窗口左上角在主屏幕左边或上边，将其移到主屏幕内
        pos_x, pos_y = self.GetPosition()
        if pos_x < primary_screen_pos[0]:
            pos_x = primary_screen_pos[0]
        if pos_y < primary_screen_pos[1]:
            pos_y = primary_screen_pos[1]
        self.SetPosition(wx.Point(pos_x, pos_y))
        
        # 如果窗口右边或下边超出了主屏幕，将其移到主屏幕内
        if pos_x + self.GetSize()[0] > primary_screen_pos[0] + primary_screen_size[0]:
            pos_x = primary_screen_pos[0] + primary_screen_size[0] - self.GetSize()[0]
        if pos_y + self.GetSize()[1] > primary_screen_pos[1] + primary_screen_size[1]:
            pos_y = primary_screen_pos[1] + primary_screen_size[1] - self.GetSize()[1]
        self.SetPosition(wx.Point(pos_x, pos_y))
        
app