可以通过 PowerShell 脚本在 Web Deploy 包中设置 ACL，以下是示例代码：

1. 创建 PowerShell 脚本 Set-WebDeployACL.ps1

Param(
[string]$PackagePath = $(throw "-PackagePath is required."),
[string]$UserName = $(throw "-UserName is required."),
[string]$Access = $(throw "-Access is required (allow|deny)."),
[string]$Permission = $(throw "-Permission is required."),
[int]$InheritanceFlags = $(throw "-InheritanceFlags is required."),
[int]$PropagationFlags = $(throw "-PropagationFlags is required.")
)

$binPath = [System.IO.Path]::GetDirectoryName($MyInvocation.MyCommand.Path)
Add-Type -Path "$binPath\Microsoft.Web.Deployment.dll"

# Open the package.
$deploymentPackageProvider = New-Object Microsoft.Web.Deployment.DeploymentProviderFactory("package")
$deploymentPackage = $deploymentPackageProvider.CreateObject("package", $PackagePath, $false)

$username = $UserName
$access = [Microsoft.Web.Deployment.SecurityDescriptorAccess]::$Access
$permission = [Microsoft.Web.Deployment.SecurityPermission]::$Permission
$inheritanceFlag = [System.Security.AccessControl.InheritanceFlags]::$InheritanceFlags
$propagationFlag = [System.Security.AccessControl.PropagationFlags]::$PropagationFlags

$securityDescriptor = New-Object Microsoft.Web.Deployment.SecurityDescriptor($username, $access, $permission, $inheritanceFlag, $propagationFlag)
$deploymentPackage.SecurityDescriptors.Add($securityDescriptor)
$deploymentPackage.SyncTo("iisApp")

此脚本使用 Microsoft.Web.Deployment.dll，该程序集是 Web Deploy 的发布程序集，要安装这个程序集，请使用以下命令：

Install-Package Microsoft.Web.Deployment -Version 3.6.0

2.编写 PowerShell 脚本设置 ACL

$packagePath = "C:\MyWebApp.deploy.cmd"
$userName = "BUILTIN\IIS_IUSRS"
$access = "Allow"
$permission = "ReadAndExecute"
$inheritanceFlags = 2
$propagationFlags = 0

& ".\Set-WebDeployACL.ps1" -PackagePath $packagePath -UserName $userName -Access $access -Permission $permission -InheritanceFlags $inheritanceFlags -PropagationFlags $propagationFlags

以上脚本会在 Web Deploy 包中设置 ACL，以便 IIS_IUSRS 用户组可以读取和执行内容。注意，这里传递给 Set-WebDeployACL.ps1 脚本的参数的值可能需要根据你的具体情况进行修改。